Creative Biolabs

Human Parkin ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP46]

Applications:
ELISA

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Description
The human Parkin ELISA kit is a single wash 90 min sandwich ELISA for the quantitative determination of Parkin protein in cell culture fluid and tissue fluid. It uses our proprietary SimpleStep ELISA® technology. Quantitative human Parkin, 9.7 pg/ml sensitivity.
Species Reactivity
Human
Applications
ELISA
Research Areas
Neurodegenerative Diseases
Pathways
Parkinson's Disease Signaling
Marker
Others
Sensitivity
9.7 pg/ml
Platform
Microplate
Detection Method
Colorimetric
Sample Type
Cell culture extracts, Tissue Extracts
Assay Type
Sandwich (quantitative)
Assay Time
1h 30m
Assay Duration
One step assay
Linear Range
46.88 pg/ml - 3000 pg/ml
Components
Components 1 x 96 tests
10X Human Parkin Capture Antibody 1 x 600µl
10X Human Parkin Detector Antibody 1 x 600µl
10X Wash Buffer PT 1 x 20ml
50X Cell Extraction Enhancer Solution 1 x 1ml
5X Cell Extraction Buffer PTR 1 x 10ml
Antibody Diluent 5BI 1 x 6ml
Human Parkin Lyophilized Recombinant Protein 2 vials
Plate Seals 1 unit
Sample Diluent NS 1 x 12ml
SimpleStep Pre-Coated 96-Well Microplate 1 unit
Stop Solution 1 x 12ml
TMB Development Solution 1 x 12ml
Storage
Store at +4°C. Please refer to protocols.
Research Use Only
For research use only
Target
Parkin
Official Name
PRKN
Full Name
parkin RBR E3 ubiquitin protein ligase
Alternative Names
AR-JP; EC 6.3.2.-; PRKN; Parkinson juvenile disease protein 2; E3 ubiquitin-protein ligase parkin; PDJ; Parkin; LPRS2; Parkinson disease protein 2
Gene ID
5071(Human);50873(Mouse)
Uniprot ID
O60260(Human);Q9WVS6(Mouse)
Neural Antibodies

Anti-PARK2 Monoclonal Antibody (N419), Unconjugated (CAT#: NRP-0322-P2112)

Anti-Parkin Antibody, prediluted (CAT#: NAB-2103-P789AM)

Mouse Anti-Human PRKN (1-437 aa) Monoclonal Antibody (Clone 7H90) (CAT#: NAB2105120SL)

Mouse Anti-Human Parkin Monoclonal Antibody (Clone A15165D), Unconjugated (CAT#: NAB201254LS)

Anti-PARK2 Polyclonal Antibody, Unconjugated (CAT#: NRP-0322-P2113)

Mouse Anti-Human PRKN (288-388 aa) Monoclonal Antibody (Clone 1H4) (CAT#: NAB2105118SL)

Mouse Anti-Parkin Monoclonal Antibody (1H4), Unconjugated (CAT#: NAB2007FY447)

Mouse Anti-PRKN Monoclonal Antibody (PRK8), Unconjugated (CAT#: NAB-0720-Z5699)

Mouse Anti-PRKN Monoclonal Antibody (Clone 6A158) (CAT#: NAB2105119SL)

Mouse Anti-PRKN Monoclonal Antibody (PRK8), Unconjugated, BSA and Azide Free (CAT#: NAB-0720-Z5716)

Neural Vectors

Human Parkinson disease (autosomal recessive, juvenile) 2, parkin (cDNA clone MGC:26491 IMAGE:4824892), complete cds (BC022014) clone, Untagged (CAT#: NEP-0621-R0100)

Lenti of Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) (PARK2), transcript variant 1 (NM_004562) ORF clone, Myc-DDK Tagged (CAT#: NEP-0621-R0079)

Lenti of Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) (PARK2), transcript variant 3 (NM_013988) ORF clone, mGFP Tagged (CAT#: NEP-0621-R0077)

Lenti of Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) (PARK2), transcript variant 2 (NM_013987) ORF clone, Myc-DDK Tagged (CAT#: NEP-0621-R0078)

Lenti of Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) (PARK2), transcript variant 2 (NM_013987) ORF clone, mGFP Tagged (CAT#: NEP-0621-R0076)

Lenti of Mouse Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_016694) ORF clone, Myc-DDK Tagged (CAT#: NEP-0621-R0074)

Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) (PARK2), transcript variant 3 (NM_013988) ORF clone/lentiviral particle, Myc-DDK Tagged (CAT#: NEP-0621-R0033)

Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) (PARK2), transcript variant 2 (NM_013987) ORF clone/lentiviral particle, Myc-DDK Tagged (CAT#: NEP-0621-R0034)

Lenti of Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) (PARK2), transcript variant 3 (NM_013988) ORF clone, Myc-DDK Tagged (CAT#: NEP-0621-R0075)

Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) clone, Untagged (CAT#: NEP-0621-R0106)

Neural Proteins & Peptides

Recombinant Human PRKN Protein (N-His), Denatured (CAT#: NPR2008PZ431)

Recombinant Human PRKN Protein (N-GST) (CAT#: NPR2008PZ423)

Kits

Human Parkin ELISA Kit [Colorimetric] (CAT#: NPP2011ZP46)

Human Parkin Antibody Pair, BSA and Azide Free [Colorimetric] (CAT#: NPP2011ZP61)

Human Parkin Antibody Pair Kit (CAT#: NPP2011ZP50)

Neural Cell Lines

Human PRKN Knockout HeLa Cell Lysate (CAT#: NCL2008ZP504)

Human PRKN Knockout HeLa Cell Line (CAT#: NCL2008ZP503)

Human PRKN Overexpressed 293T Cell Lysate (CAT#: NCL2008ZP501)

Parkin Mitochondrial Recruitment Assay Cell Line (CAT#: NCL2110P248)

For Research Use Only. Not For Clinical Use.
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