Human TTC3 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP331]

Human TTC3 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This is a HeLa cell transfected lysate in which Human TTC3 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurogenesis

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

TTC3

Official Name

TTC3

Alternative Names

Tetratricopeptide Repeat Domain 3; RING-Type E3 Ubiquitin Transferase TTC3; Tetratricopeptide Repeat Protein 3; RING Finger Protein 105; TPR Repeat Protein 3; TPR Repeat Protein D; RNF105;

Publications