Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric), Mammalian

Description

Monoacylglycerol lipase (MAGL, E.C.3.1.1.23) is an important enzyme that regulates endocannabinoid signaling in human physiology. MAGL is a serine hydrolase that can generate free fatty acids and glycerol from monoacylglycerols (such as 2-arachidonic glycerol (2-AG), one of the main endocannabinoids active in the nervous system). Arachidonic acid, a free fatty acid released by MAGL activity, is the precursor of eicosanoids, which are a family of pro-inflammatory signaling molecules. The remaining part of glycerol can also be used as the basis of metabolism and energy. The inhibition or inactivation of MAGL leads to accumulation of 2-AG, which has multiple biological consequences. Inhibition of the analgesic effects of Parkinson's disease and Alzheimer's disease, anti-inflammatory and neuroprotective effects, and reduction of addiction withdrawal symptoms are part of the results of MAGL inhibition. In addition, the downstream eicosanoid signal pathway is affected; this pathway plays a role in a variety of physiological processes, including inflammation, immunity, nociception, and blood pressure. As the central node that regulates lipid signaling and energy mobilization, MAGL has attracted great interest as a therapeutic target. Our monoacylglycerol lipase activity assay kit provides a fast, sensitive and simple method to measure MAGL activity in various samples. In this assay, the fluorescent substrate is cleaved to generate arachidonic acid and fluorescent metabolites, and the increased fluorescence is measured at Ex/Em 360/460 nm. In order to identify the signal specifically produced by MAGL, a specific inhibitor is included, allowing users to distinguish MAGL activity from other fluorescent sources. This assay is easy to operate, high-throughput adaptable, and can detect MAGL activity as low as 0.1 mU
Measurement of monoacylglycerol lipase activity in various tissues/cells.

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Fluoroescence (Ex/Em = 360/460 nm)

Components

• MAGL Assay Buffer
• MAGL Substrate
• MAGL Positive Control
• MAGL Selective Inhibitor (200X)
• Umbelliferone Standard (10 mM)

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

Monoacylglycerol Lipase

Official Name

MGL

Full Name

Monoacylglycerol lipase

Alternative Names

MAGL; MGL; MGLL

Gene ID

11343(Human); 23945(Mouse); 29254(Rat)

Uniprot ID

Q99685(Human); O35678(Mouse); Q8R431(Rat)