Myeloperoxidase (MPO) Fluorometric Activity Assay Kit, Mammalian

Description

Myeloperoxidase (MPO) is the most abundant peroxidase in neutrophils (EC 1.11.1.7). It is green hemoglobin found in neutrophils and monocytes. It catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates. These intermediates are oxygen-dependent to kill tumor cells and Play a role in microorganisms. Its heme pigment makes secretions rich in neutrophils (such as pus and some forms of mucus) green. Furthermore, hydrogen peroxide can be used as an oxidizing agent to oxidize tyrosine to a tyrosine group. In our MPO analysis kit, MPO catalyzes the production of NaClO from H 2 O 2 and NaCl. Subsequently, NaClO will react with aminophenylfluorescein (APF) stoichiometrically to produce fluorescein, which has strong fluorescence and can be detected at Ex / Em = 485/525 nm. The kit provides a fast, simple, sensitive and reliable test method, which is suitable as a high-throughput detection method for MPO activity. The kit can be used to detect MPO activity as low as 0.5μU per well.

Species Reactivity

Mammalian

Application Notes

Sensitive assays for measuring MPO as low as 0.5 μU per well

Relevant Diseases

Neurological Disorders

Detection Method

Fluorescence (Ex/Em = 485/525 nm)

Components

• Assay Buffer
• MPO Substrate Stock
• MPO Probe
• Fluorescein Standard (1 mM)
• MPO Positive Control

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

Myeloperoxidase

Official Name

MPO

Full Name

Myeloperoxidase

Alternative Names

Myeloperoxidase; EC 1.11.1.7; EC 1.11.2.2; EC 1.11.1

Gene ID

4353(Human); 17523(Mouse); 303413(Rat)

Uniprot ID

P05164(Human); P11247(Mouse)