NeuroMab™ Anti-NGF Antibody,Clone NR1963P

Figure 1 is a graph comparing the NGF blocking effect of various Fabs in the presence of human NGF 0.04 ng/ml.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

Figure 2 is a graph showing the results of BIAcore analysis of human NGF binding affinity of mouse antibody 911 (Fab). Mouse antibody 911 binds NGF with a KD of 3.7 nM, a koff of 8.4 x 10-5 s-1 and a kon of 2.2 x 104 Ms-1.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

Figure 3 is a graph showing the results of BIAcore analysis of human NGF binding affinity of E3 (Fab) antibody (referred to as "Fab 3E"). E3 binds to human NGF with a KD of about 0.07 nM (kon about 6.0 x 105 M-1 s-1, koff about 4.2 x 10-5 s-1).

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

Figure 4 is a graph showing that E3 antibody blocks the interaction of NGF with its receptors trkA and p75.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

Figure 5 is a graph showing the capacities of Antibody E3 (solid triangles; referred to as "3E"), Antibody 911 (solid circles) at different concentrations (20, 4, 0.8, 0.16, 0.032, 0.0064, 0.00128, and 0.0 nM) and The trkA receptor immunoadhesin (shaded squares; designated 'trkA-Fc') inhibits NGF-dependent survival of trigeminal neurons E13.5.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

Figure 6 is a graph showing the nociceptive response of arthritic rats (rheumatoid arthritis model) after administration of anti-NGF antibodies (E3 and 911) on D14 and D19.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).

Figure 7 is a graph showing the effect of anti-NGF antibody on body weight of arthritic rats (rheumatoid arthritis model) after administration of anti-NGF antibody on D14 and D19.

Axis corresponds to concentration of 3E antibody (Fab) and Y axis corresponds to NGF binding (percentage of maximal UR). Increasing concentrations of Fab E3 blocked the interaction of NGF with p75 and trkA, as indicated by a decrease in signal (measured in UR). When the concentration of E3 antibody (Fab) was equal to that of NGF, no NGF binding was observed (indicated by zero signal).