Mouse Anti-NFKB1 Monoclonal Antibody (CBP2883)

Description

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity.

Expression Host

Mammalian cells

Immunogen

NFKB1 fusion protein Ag5832

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2883

Applications

IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

NFKB1

Official Name

NFKB1

Full Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Alternative Names

DNA binding factor KBF1;
DNA binding factor KBF1 EBP1;
DNA-binding factor KBF1;
EBP 1;
EBP-1;
EBP1;
KBF1;

Gene ID

4790(Human); 18033(Mouse); 81736(Rat)

Uniprot ID

P19838(Human); P25799(Mouse); Q63369(Rat)

1. Wang, M., Wang, Z., Zhu, X., Guan, S., & Liu, Z. (2019). NFKB1-miR-612-FAIM2 pathway regulates tumorigenesis in neurofibromatosis type 1. In Vitro Cellular & Developmental Biology-Animal, 55(7), 491-500. 2. Yang, D., Zhang, W., Liang, J., Ma, K., Chen, P., Lu, D., & Hao, W. (2018). Single cell whole genome sequencing reveals that NFKB1 mutation affects radiotherapy sensitivity in cervical cancer. Oncotarget, 9(7), 7332.