Mouse Anti-mCherry Monoclonal Antibody (CBP6633)

Description

The mCherry protein is derived from the natural product DsRed, which was originally isolated as red fluorescent protein from corals of the genus Discosoma (1). Like other natural fluorescent proteins in cnidarians (jellyfish, sea anemones, and corals), the natural form of this protein forms a stable tetramer in the body. In the Tsien laboratory, DsRed aims to improve its spectral properties and prevent multimerization, where a lot of work on fluorescent proteins has been done (2). Roger Tsien, Martin Chalfie and Osamyu Shinomura won the 2008 Nobel Prize in Chemistry for the discovery and development of cnidarian fluorescent protein. Several further cycles of mutation, directed modification, and evolutionary selection produced mCherry, which is a monomer with maximum excitation at 587nm and maximum emission at 610nm (3). The protein is widely used as a fluorescent tracer in transfection, genetic modification, photobleaching, and FRET type experiments. The prototype of these fluorescent proteins is Green Fluorescent Protein (GFP), which is a protein of approximately 27 kDa, originally isolated from the jellyfish Aequoria victoria (4). The mCherry protein is similar to GFP (5,6) in size and general structural properties, but apparently produces a red rather than green fluorescent dye. Like GFP, mCherry only needs molecular oxygen to become fluorescent due to its intrinsic properties, so it can be easily expressed in various systems.

Immunogen

Full-length recombinant protein

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP6633

Applications

WB; IF; IHC

Application Notes

WB: 1:2000
IF/IHC: 1:500

Form

Liquid

Formulation

50% PBS, 50% glycerol

Preservatives

5mM NaN3

Concentration

1 mg/ml

Shipping

Gel Pack

Storage

Stable at 4°C for one year, for longer term store at -20°C

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

mCherry

Official Name

D1MPT3

Alternative Names

D1MPT3; mCherry