Creative Biolabs

Nitrotyrosine ELISA Kit, Species Independent

[CAT#: NK1120FY010]

Colorimetric detection of Nitrotyrosine

Applications:
Competitive ELISA (Enzyme-linked Immunosorbent Assay)

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Description
1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
Species Reactivity
Wide Species
Applications
Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Relevant Diseases
Alzheimer's Disease; Parkinson's Disease
Research Areas
Neurodegeneration
Sensitivity
50 nM
Platform
Microplate
Detection Method
Colorimetric Assay
Sample Type
Cell lysates, Plasma, Serum, Urine
Assay Type
ELISA Kits
Assay Time
1 Hour
Precision
Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
Number Of Samples
38 samples in duplicate
Linear Range
62.5 - 8000 nM
Components
Nitrosylated BSA Coated Plate 1 Plate
SNitrotyrosine Standard 1 vial/110µl
Nitrotyrosine HRP Conjugated Monoclonal Antibody 1 vial/75µl
Sample and Standard Diluent (Red) 1 vial/50mL
Nitrotyrosine Antibody Diluent (Blue) 1 vial/13mL
Wash Buffer Concentrate (10X) 1 vial/ 50mL
TMB Substrate 1 vial/13mL
Stop Solution 1 vial/13mL
Plate Cover 2
Shipping
Blue Ice
Storage
4°C
Research Use Only
For research use only
Target
Nitro tyrosine
Official Name
Nitrotyrosine
For Research Use Only. Not For Clinical Use.
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