Rat Peroxisome Proliferator-activated receptor γ, PPAR-γ ELISA Kit

[CAT#: NRZP-0722-ZP272]

For the quantitative determination of rat peroxisome proliferator-activated receptor γ (PPAR-γ) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of rat PPAR-γ.

Species Reactivity

Rat

Marker

Peroxisome proliferator-activated receptor gamma

Properites

Sensitivity

In most cases, the sensitivity is below 0.039ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.156 ng/ml-10 ng/ml

Max Emission

450 nm

Target Details

Target

PPARG

Official Name

PPARG

Full Name

peroxisome proliferator activated receptor gamma

Alternative Names

GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma

Gene ID

25664(Rat)

Uniprot ID

O88275(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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