Product
Beta-Secretase (BACE1) Inhibitor Screening Kit (Fluorometric), Human
[CAT#: NK1120FY264]
The assay is sensitive, stable and high-throughput adaptable.
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
β-secretase (β-position amyloid precursor protein (APP) cleaving enzyme 1 / BACE1) participates in the initial cleavage of amyloid precursor-APP, generating two soluble N-terminal and C-terminal fragments. Another secretase isoenzyme, Secretase, cleaves the C-terminal fragment to produce amyloid β. The accumulation of amyloid in the brain leads to the formation of amyloid plaques, which leads to the development of Alzheimer's disease (AD) in humans. Therefore, in Alzheimer's disease-related drug discovery research, the screening of new and specific human β-secretase inhibitors (BACE1) has become crucial. In the human β-secretase (BACE1) inhibitor screening kit for the determination of Genie, human BACE1 cleaves the BACE1 specific quenching substrate to produce a product with high fluorescence, which can be tested at Ex / Em = 435/500 Measured at nm. In the presence of a BACE1 inhibitor (control included in the kit), enzymatic cleavage is hindered and can be used to screen new inhibitor compounds.
Application Notes
Screening/characterizing/studying potential inhibitors of Human Beta-Secretase (BACE1)
Relevant Diseases
Neurological Disorders
Properites
Detection Method
Fluorescence (Ex/Em= 345/500 nm)
Assay Type
Inhibitor Screening Kit
Assay Time
Less than 1 hr
Components
BACE1 Assay Buffer
BACE1 Substrate (in DMSO)
BACE1
BACE1 Inhibitor Control (in DMSO)
BACE1 Substrate (in DMSO)
BACE1
BACE1 Inhibitor Control (in DMSO)
Preparation and Storage
Shipping
Gel Pack
Storage
-20°C
Handling Advices
The antibody solution should be gently mixed before use.
Research Use Only
For research use only
Target Details
Target
BACE1
Official Name
BACE1
Full Name
Beta-Secretase 1
Alternative Names
Beta-Secretase 1; Membrane-Associated Aspartic Protease 2; Beta-Site APP Cleaving Enzyme 1; Beta-Site APP-Cleaving Enzyme; Aspartyl Protease 2; EC 3.4.23.46; Memapsin-2; Asp 2; BACE; ASP2
Data
Figure 1 Progress Curves of BACE Enzyme Control and Inhibitor Control. BACE1 activity was inhibited more than 95% following kit protocols and using Inhibitor Control.
Figure 2 Inhibition of Human β-Secretase (BACE1) activity by BACE1 Inhibitor (IC 50 = 1 nM). Assay was performed following the kit protocol.
Figure 2 Inhibition of Human β-Secretase (BACE1) activity by BACE1 Inhibitor (IC 50 = 1 nM). Assay was performed following the kit protocol.
Publications
Publications (0)
Neural Genes
Kits
Neural Antibodies
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
