Human ADAM17 Knockout HCT116 Cell Lysate

[CAT#: NCL2008ZP588]

Human ADAM17 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HCT116 cell transfected lysate in which Human ADAM17 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon5.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration

Marker

Tangles & Tau

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 10, 11
D13S317: 10, 12
D7S820: 11, 12
D16S539: 11, 13
vWA: 17, 22
TH01: 8,9
TPOX: 8, 9
CSF1PO: 7, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

ADAM17

Official Name

ADAM17

Full Name

ADAM Metallopeptidase Domain 17

Alternative Names

ADAM Metallopeptidase Domain 17; Tumor Necrosis Factor; Alpha; Converting Enzyme; Snake Venom-Like Protease; TNF-Alpha Convertase; EC 3.4.24.86; TACE; CSVP; Disintegrin And Metalloproteinase Domain-Containing Protein 17; ADAM Metallopeptidase Domain 18;