Creative Biolabs

Human ADAM17 Knockout HCT116 Cell Lysate

[CAT#: NCL2008ZP588]

Human ADAM17 knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HCT116 Cell

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Description
This is a HCT116 cell transfected lysate in which Human ADAM17 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HCT116 Cell
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon5.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 10, 11
D13S317: 10, 12
D7S820: 11, 12
D16S539: 11, 13
vWA: 17, 22
TH01: 8,9
TPOX: 8, 9
CSF1PO: 7, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
ADAM17
Official Name
ADAM17
Full Name
ADAM Metallopeptidase Domain 17
Alternative Names
ADAM Metallopeptidase Domain 17; Tumor Necrosis Factor; Alpha; Converting Enzyme; Snake Venom-Like Protease; TNF-Alpha Convertase; EC 3.4.24.86; TACE; CSVP; Disintegrin And Metalloproteinase Domain-Containing Protein 17; ADAM Metallopeptidase Domain 18;
Gene ID
6868 (Human); 11491 (Mouse)
Uniprot ID
P78536 (Human); Q9Z0F8 (Mouse)
For Research Use Only. Not For Clinical Use.
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