Human ATG16L1 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP510]

Human ATG16L1 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human ATG16L1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon1 and Insertion of the selection cassette in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neurodegeneration

Species

Human

Properties

Form

There are 4 isoforms produced by alternative splicing.

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

ATG16L1

Official Name

ATG16L1

Full Name

Autophagy Related 16 Like 1

Alternative Names

Autophagy Related 16 Like 1; APG16L; ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae); ATG16 Autophagy Related 16-Like (S. Cerevisiae); APG16 Autophagy 16-Like (S. Cerevisiae); ATG16 Autophagy Related 16-Like 1; Autophagy-Related Protein 16-1; WD Repeat Domain 30;