Creative Biolabs

Human FTH1 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP574]

Human FTH1 knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HEK 293T

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Description
This is a HEK293T cell transfected lysate in which Human FTH1 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HEK 293T
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 1.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Species
Human
Cell State
Cell lysate
Tissue Source
Kidney
Cell Purity
>95%
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Ferritin Heavy Chain
Official Name
FTH1
Full Name
Ferritin Heavy Chain 1
Alternative Names
Ferritin Heavy Chain 1; Cell Proliferation-Inducing Gene 15 Protein; Proliferation-Inducing Protein 15; Placenta Immunoregulatory Factor; Ferritin; Heavy Polypeptide 1; Ferritin H Subunit; Apoferritin; EC 1.16.3.1;
Gene ID
2495(Human); 14319(Mouse); 25319(Rat)
Uniprot ID
P02794(Human); P09528(Mouse); P19132(Rat)
For Research Use Only. Not For Clinical Use.
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