Human FYN Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP576]

Human FYN knockout cell lysate

Species:: Human

Cell Types:: HEK293T

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human FYN has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK293T

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration

Marker

Tangles & Tau

Species

Human

Properties

Form

This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

Fyn

Official Name

FYN

Full Name

FYN Proto-Oncogene, Src Family Tyrosine Kinase

Alternative Names

FYN Proto-Oncogene; Src Family Tyrosine Kinase; FYN Oncogene Related To SRC; FGR; YES; Proto-Oncogene C-Fyn; Proto-Oncogene Syn; Src-Like Kinase; EC 2.7.10.2; P59-FYN; SLK;