Product
Human GSK-3beta (pS9 / Total) ELISA Kit [Colorimetric]
[CAT#: NPP2011ZP70]
- Applications:
- ELISA
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
ELISA (enzyme-linked immunosorbent assay) kit is used for semi-quantitative determination of GSK-3 protein (pS9) and total GSK-3 protein (Total) in human and mouse cells. Glycogen synthase kinase-3 (GSK3) was originally identified as an enzyme that phosphorylates and inactivates glycogen synthase in response to insulin to regulate glycogen synthesis. GSK3 is expressed as GSK3A and GSK3B, two closely related and similarly regulated isoforms. It is known that GSK3B participates in a variety of cell signal transduction processes, including glycogen synthesis, cell adhesion, and is related to Alzheimer's disease. GSK3B is an important element of PI3 kinase/Akt signaling pathway, and its kinase activity is down-regulated by Akt-mediated phosphorylation of Ser9
Species Reactivity
Human
Applications
ELISA
Research Areas
Neural Signal Transduction
Pathways
Notch Pathway
Properites
Sensitivity
5 µg/ml
Platform
Microplate
Detection Method
Colorimetric
Sample Type
Cell culture extracts, Cell Lysate
Assay Type
Semi-quantitative
Assay Time
1h 30m
Assay Duration
One step assay
Components
Components
Plate Seal 1 x unit
SimpleStep Pre-Coated 96 Well Microplate (12 x 8 well strips) 1 x 96test
Stop Solution 1 x 12ml
10X Wash Buffer PT 1 x 15ml
5X Cell Extraction Buffer PTR 1 x 10ml
50X Cell Extraction Enhancer Solution 1 x 1ml
TMB Substrate 1 x 12ml
Lyophilized GSK-3β Control Lysate 1 x 1vial
GSK-3β (Total) Capture Antibody 1 x 3ml
GSK-3β (Total) Detector Antibody 1 x 3ml
GSK-3β (pS9) Capture Antibody 1 x 3ml
GSK-3β (pS9) Detector Antibody 1 x 3ml
Plate Seal 1 x unit
SimpleStep Pre-Coated 96 Well Microplate (12 x 8 well strips) 1 x 96test
Stop Solution 1 x 12ml
10X Wash Buffer PT 1 x 15ml
5X Cell Extraction Buffer PTR 1 x 10ml
50X Cell Extraction Enhancer Solution 1 x 1ml
TMB Substrate 1 x 12ml
Lyophilized GSK-3β Control Lysate 1 x 1vial
GSK-3β (Total) Capture Antibody 1 x 3ml
GSK-3β (Total) Detector Antibody 1 x 3ml
GSK-3β (pS9) Capture Antibody 1 x 3ml
GSK-3β (pS9) Detector Antibody 1 x 3ml
Preparation and Storage
Storage
Store at +4°C. Please refer to protocols.
Research Use Only
For research use only
Target Details
Publications
Publications (0)
Neural Antibodies
Kits
For Research Use Only. Not For Clinical Use.
