Human KCNN4 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP187]

Human KCNN4 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human KCNN4 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 80 bp insertion in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Potassium Signaling

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

KCNN4

Official Name

KCNN4

Full Name

Potassium Calcium-Activated Channel Subfamily N Member 4

Alternative Names

Potassium Calcium-Activated Channel Subfamily N Member 4; Potassium Channel; Calcium Activated Intermediate/Small Conductance Subfamily N Alpha; Member 4; Potassium Intermediate/Small Conductance Calcium-Activated Channel; Subfamily N; Member 4; Small Conductance Calcium-Activated Potassium Channel 4; Putative Gardos Channel; SKCa 4; KCa3.1; IKCA1; SKCa4; KCA4; IK1;