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Creative Biolabs
Product

Human PEDF ELISA Kit (SerpinF1) [Colorimetric]

[CAT#: NPP2011ZP129]

Applications:
ELISA

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Product Overview

Description

Human PEDF enzyme-linked immunosorbent assay (ELISA) kit (SerpinF1) is used to quantitatively detect human PEDF in cell culture supernatant, cell lysate, tissue homogenate, serum and plasma (heparin, EDTA).

Species Reactivity

Human

Applications

ELISA

Research Areas

Neurogenesis

Pathways

Neurogenesis Signaling
Properites

Sensitivity

< 20 pg/ml

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

Assay Type

Sandwich (quantitative)

Assay Time

3h 30m

Assay Duration

Multiple steps standard assay

Linear Range

0.78 ng/ml - 50 ng/ml

Components

Components Identifier 1 x 96 tests
ABC Diluent Buffer Blue Cap 1 x 12ml
Adhesive Plate Seal 4 units
Antibody Diluent Buffer Green Cap 1 x 12ml
Anti-Human PEDF coated Microplate (12 x 8 wells) 1 unit
Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
Biotinylated anti-Human PEDF antibody 1 x 130µl
Lyophilized recombinant Human PEDF standard 2 vials
Sample Diluent Buffer Green Cap 1 x 30ml
TMB Color Developing Agent Black Cap 1 x 10ml
TMB Stop Solution Yellow Cap 1 x 10ml
Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only
Target Details

Target

PEDF

Official Name

SERPINF1

Full Name

serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

Alternative Names

OI12; Pigment epithelium-derived factor; Cell proliferation-inducing gene 35 protein; Serpin F1; EPC-1; PEDF; PIG35; OI6

Gene ID

5176(Human);20317(Mouse)

Uniprot ID

P36955(Human);P97298(Mouse)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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