Human U-251 MG Glioblastoma Cell Line

Species:: Human

Cell Types:: Tumor Cells

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Product Overview

Cell Types

Tumor Cells

Application Notes

Split 1:2 or 1:3 every 3 days using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37C.

Relevant Diseases

Glioblastoma

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen

Growth Pattern

Fibroblastic

Freeze Medium

90% (MEM Zinc Option with 20% FBS) + 10% DMSO

Biosafety Level

BSL2

Tissue Source

Brain

Shipping

Dry ice

Storage

Liquid Nitrogen

Research Use Only

This product is intended for research use only.

Donor Information

Donor Health

Tumor

Publications

Publications (0)

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Case Studies

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Excellent consistency

The U-251 MG glioblastoma cell line has been a staple in our lab for glioma research. The cells exhibit consistent growth patterns, and their response to treatment protocols has been reproducible across multiple experiments. This consistency is critical for our studies on therapeutic efficacy.

Excellent

Reliable for drug screening

We used the U-251 MG cell line for high-throughput drug screening. The cells adapted well to different drug concentrations, and the viability assays produced clear, reliable results. This line is ideal for testing novel chemotherapeutic agents.

Excellent

High transfection efficiency

Transfection efficiency with the U-251 MG cells has been outstanding. We've successfully introduced various plasmids, and the cells have shown robust expression of transgenes. This has significantly accelerated our research on gene therapy for glioblastoma.

Excellent

Good morphological characteristics

The U-251 MG cell line exhibits typical glioblastoma morphology, which has been very helpful for our imaging studies. The cells are easy to culture, and their growth rate is manageable, allowing for detailed, time-lapse studies of cellular behavior.

Excellent

Responsive to treatment

We've found the U-251 MG cells to be highly responsive to various treatment protocols, particularly in apoptosis and cell viability assays. This responsiveness has made it easier to draw meaningful conclusions from our experiments.

Q&As

What transfection methods work best with U-251 MG cells?

U-251 MG cells are highly amenable to transfection, and a variety of methods can be used effectively, including lipofection, electroporation, and viral transduction. Lipofection is particularly popular due to its simplicity and high efficiency. We recommend optimizing the transfection protocol by adjusting DNA/RNA concentrations and transfection reagent volumes to achieve the best results for your specific experimental needs.

Are there any specific markers that U-251 MG cells express that are useful for research?

Yes, the U-251 MG glioblastoma cell line expresses several key markers that are highly relevant for glioblastoma research. These include Glial Fibrillary Acidic Protein (GFAP), which is a marker for astrocytes, and other markers associated with glioma such as EGFR and MMP-2. These markers are useful for studying glioblastoma biology, tumor progression, and the cellular response to various treatments.

How should I handle and store the U-251 MG cell line upon receipt?

Upon receipt, the U-251 MG cell line should be thawed and cultured immediately. If immediate use is not possible, the cells can be stored in liquid nitrogen for long-term preservation. When thawing, we recommend using a 37°C water bath, followed by gentle resuspension in pre-warmed growth medium. It's critical to avoid prolonged exposure to the water bath to prevent cell damage. Once thawed, cells should be centrifuged and resuspended in fresh medium to remove cryoprotectant before seeding into culture flasks.