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Creative Biolabs

Human Corneal Epithelial Cell Line HCE-T

[CAT#: NCL-2105-P259-AM] Review(5) Q&As(3)

Species:
Human
Cell Types:
Visual system-related Cells

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Product Overview

Cell Types

Visual system-related Cells

Species

Human
Properties

Culture Medium

DMEM/F12+ 15% FBS+ 5μg/ml Insulin + 10ng/ml human EGF+1% P/S

Freeze Medium

55% basic medium + 40% FBS + 5% DMSO

STR Analysis

Pass

Tissue Source

Eye

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Negative

Sterility Testing

The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen on dry ice

Storage

Liquid nitrogen immediately upon delivery

Research Use Only

For research use only

Quality Control

1) Scanning/transmission electron microscopy analysis; 2) Immunocytochemistry; 3) Reverse transcription polymerase chain reaction; 4) Western blotting; 5) Scratch/proliferation/migration test
Donor Information

Gender

Female
Publications

Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
Ideal for corneal drug permeability studies
We’ve used this cell line extensively for corneal drug permeability testing. The cells are robust and mimic human corneal epithelial properties effectively. The tight junctions formed make it an ideal model for barrier studies. The doubling time is reasonable, and the cells are easy to culture, which is a plus.
user
Excellent
Reliable and consistent
The HCE-T cell line has been our go-to model for studying corneal epithelial dynamics. The cells consistently grow in a monolayer and maintain a healthy phenotype over multiple passages. We’ve used it for both gene expression analysis and drug testing, and the results have been reproducible and reliable.
user
Excellent
User-friendly and cost-effective
For labs on a budget, this cell line is an excellent option. It’s easy to culture and requires minimal specialized media supplements. We’ve achieved consistent growth curves and gene expression profiles over several batches.
user
Excellent
High-quality cell line for toxicity testing
The HCE-T cell line has been useful in our corneal toxicity tests for new ophthalmic drugs. The cells grow uniformly and respond predictably to toxic compounds, allowing us to generate clear dose-response curves. We’ve been impressed with the reproducibility across different batches of cells.
user
Excellent
Stable and predictable growth
We appreciate how easy it is to maintain the HCE-T cells. They grow in a predictable manner with stable doubling times, which is critical when planning experiments. The cells maintain their epithelial morphology well, even after extended culture, making them reliable for long-term studies.
Q&As
How often should I pass the HCE-T cells?
The HCE-T cells should be subcultured every 3 to 5 days, depending on the growth rate. You can monitor the confluence of the culture; once the cells reach 70-80% confluence, it’s time to subculture. Frequent passaging helps maintain cell health and prevents overgrowth, which can lead to changes in cell characteristics.
How can I assess the functionality of the HCE-T cells in my experiments?
Assess cell functionality using assays such as barrier integrity measurements (e.g., TEER), cellular morphology analysis, and gene expression studies. Immunofluorescence staining for epithelial markers can also help confirm cell phenotype. Functional assays should be tailored to your specific research goals.
How long can I keep the HCE-T cells in culture before they need to be discarded?
The HCE-T cells can generally be kept in culture for up to 15-20 passages, though this may vary depending on your specific experimental conditions and culture practices. Regular monitoring of cell morphology and growth characteristics will help determine when it’s time to subculture or replace the cell line to maintain experimental consistency.
For Research Use Only. Not For Clinical Use.
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