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- Species:
- Human
- Cell Types:
- Neural Stem Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the P301L mutation (CCG>CTG) into the MAPT gene. This line is homozygous for the P301L mutation so both alleles contain the mutation. Click on the product images to see the data and further details.
The P301L mutation MAPT has been implicated in frontotemporal dementia and parkinsonism (Dumanchin et al., 1998). The mutation affects only the 4R isoforms of MAPT since the exon containing the mutation is spliced out of 3R isoforms (Hutton et al., 1998). Aggregated MAPT/tau protein in affected patients consisted mainly of the 4R isoform (Hutton et al., 1998). The P301L mutation promotes aggregation of MAPT/tau protein into ordered paired helical filaments and beta sheet formation in vitro (von Bergen et al., 2001).
The P301L mutation MAPT has been implicated in frontotemporal dementia and parkinsonism (Dumanchin et al., 1998). The mutation affects only the 4R isoforms of MAPT since the exon containing the mutation is spliced out of 3R isoforms (Hutton et al., 1998). Aggregated MAPT/tau protein in affected patients consisted mainly of the 4R isoform (Hutton et al., 1998). The P301L mutation promotes aggregation of MAPT/tau protein into ordered paired helical filaments and beta sheet formation in vitro (von Bergen et al., 2001).
Neurological Disease Models
AD-related Cells
Cell Types
Neural Stem Cells
Relevant Diseases
Alzheimer's Disease
Species
Human
Properties
Size
1.5 million cells & Neural Plating-XF Medium
Growth Pattern
Adherent
Tissue Source
Fibroblasts (64 Yr Donor)
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
Donor Information
Donor Health
Disease
Publications
Publications (0)
Neural Cell Lines
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentThese cells are incredibly robust and have shown consistent expression of the P301L mutationTheir differentiation into mature neurons is efficient, and they exhibit hallmark features of tau aggregation. The cells' viability over long culture periods has been impressive, making them an invaluable tool for our neurodegenerative disease studies.
ExcellentThe cells are easy to culture and maintain, and they differentiate into neurons with high efficiencyThe presence of the MAPT P301L mutation provides a reliable model for studying tau pathology, and we have observed clear tau tangles in our assays. Highly recommend these cells for anyone studying tau-related disorders.
ExcellentFor modeling tau-induced neurodegenerationThe cells are consistently healthy and proliferate well, allowing for repeated experiments. The differentiation process is straightforward, and the resulting neurons display characteristics typical of tauopathy.
ExcellentThe cells are highly reliable, and their differentiation into neurons is both rapid and efficientThe MAPT P301L mutation is expressed robustly, providing an excellent model for studying tauopathies. These cells have greatly facilitated our understanding of tau-related mechanisms in neurodegenerative diseases.
ExcellentEasy to handleWe have observed significant tau pathology in our models. The quality and reliability of these cells are top-notch, making them an essential tool for our research.
Q&As
Can these neural stem cells differentiate into multiple neural lineages?
Yes, the cells are pluripotent and capable of differentiating into various neural lineages, including neurons, astrocytes, and oligodendrocytes. This characteristic allows researchers to model diverse aspects of neurodegenerative diseases and investigate how the MAPT P301L mutation affects different cell types in the central nervous system.
How long can the cells be passaged before their functionality is compromised?
The cells can be passaged multiple times; however, it is recommended to use them within 15-20 passages to ensure consistent functionality and quality. Prolonged passaging may lead to changes in cell behavior and differentiation potential, so routine assessment of cell characteristics is advised.
How do the MAPT P301L HOM cells compare to wild-type neural stem cells in terms of behavior and function?
MAPT P301L HOM cells exhibit characteristics associated with tauopathies, such as abnormal tau phosphorylation and aggregation, which are not present in wild-type neural stem cells. These differences allow researchers to investigate disease-specific mechanisms and identify potential targets for intervention. Comparative studies can provide insights into how the MAPT P301L mutation alters cellular behavior and contributes to neurodegeneration.
For Research Use Only. Not For Clinical Use.
