iNeu™ Human Neural Stem Cells, MAPT P301L HOM

Species:: Human

Cell Types:: Neural Stem Cells

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Product Overview

Neurological Disease Models

AD-related Cells

Cell Types

Neural Stem Cells

Relevant Diseases

Alzheimer's Disease

Species

Human

Properties

Size

1.5 million cells & Neural Plating-XF Medium

Growth Pattern

Adherent

Tissue Source

Fibroblasts (64 Yr Donor)

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.

Donor Information

Donor Health

Disease

Publications

Publications (0)

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Case Studies

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

These cells are incredibly robust and have shown consistent expression of the P301L mutation

Their differentiation into mature neurons is efficient, and they exhibit hallmark features of tau aggregation. The cells' viability over long culture periods has been impressive, making them an invaluable tool for our neurodegenerative disease studies.

Excellent

The cells are easy to culture and maintain, and they differentiate into neurons with high efficiency

The presence of the MAPT P301L mutation provides a reliable model for studying tau pathology, and we have observed clear tau tangles in our assays. Highly recommend these cells for anyone studying tau-related disorders.

Excellent

For modeling tau-induced neurodegeneration

The cells are consistently healthy and proliferate well, allowing for repeated experiments. The differentiation process is straightforward, and the resulting neurons display characteristics typical of tauopathy.

Excellent

The cells are highly reliable, and their differentiation into neurons is both rapid and efficient

The MAPT P301L mutation is expressed robustly, providing an excellent model for studying tauopathies. These cells have greatly facilitated our understanding of tau-related mechanisms in neurodegenerative diseases.

Excellent

Easy to handle

We have observed significant tau pathology in our models. The quality and reliability of these cells are top-notch, making them an essential tool for our research.

Q&As

Can these neural stem cells differentiate into multiple neural lineages?

Yes, the cells are pluripotent and capable of differentiating into various neural lineages, including neurons, astrocytes, and oligodendrocytes. This characteristic allows researchers to model diverse aspects of neurodegenerative diseases and investigate how the MAPT P301L mutation affects different cell types in the central nervous system.

How long can the cells be passaged before their functionality is compromised?

The cells can be passaged multiple times; however, it is recommended to use them within 15-20 passages to ensure consistent functionality and quality. Prolonged passaging may lead to changes in cell behavior and differentiation potential, so routine assessment of cell characteristics is advised.

How do the MAPT P301L HOM cells compare to wild-type neural stem cells in terms of behavior and function?

MAPT P301L HOM cells exhibit characteristics associated with tauopathies, such as abnormal tau phosphorylation and aggregation, which are not present in wild-type neural stem cells. These differences allow researchers to investigate disease-specific mechanisms and identify potential targets for intervention. Comparative studies can provide insights into how the MAPT P301L mutation alters cellular behavior and contributes to neurodegeneration.