Mouse Cementocyte-Like Cell Line (IDG-CM6)

[CAT#: NCL-2106-S31]

Clonal cementocyte-like cell line.

Species:: Mouse

Cell Types:: Tooth-related Cells

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Product Overview

Description

This product at Creative Biolabs can be expanded under permissive conditions, at 33°C in the presence of IFN-γ, and then allowed to resume their original phenotype, at 37°C in the absence of IFN-γ.

Cell Types

Tooth-related Cells

Application Notes

Under permissive conditions at 33C, when at 80-90% confluent, use a 0.05% Trypsin/ 0.53mM EDTA solution to detach cells. Split 1:5 to 1:10 based on your need. Cells grow best when cultured at higher densities; at low densities, proliferation can be slow. Exchange media every 2-3 days.

Species

Mouse

Properties

Size

>1x10^6 Cells

Form

Frozen

Growth Pattern

Adherent

Culture Medium

Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; penicillin-streptomycin at 100U/ml-100ug/ml; Recombinant Mouse Interferon-gamma (INF-g) range 8-50 U/ml; for growing at 33°C.
Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; penicillin-streptomycin at 100U/ml-100ug/ml; approximately 50µg/ml Ascorbic Acid and 4mM β-glycerophosphate; for growing at 37°C.

Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen.

Freeze Medium

60% AlphaMEM, 30% FBS, 10% DMSO, at 1-2 x 10^6 cells, vial, 1ml

Biosafety Level

BSL1

Tissue Source

Apical portion of tooth roots from 1st to 3rd mandibular molars

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

For experimental purposes, plate the cells at a density of 4x10^4 cells/cm2, in proliferation medium and incubate at 33C, 5% CO2 , until confluent, called Day 0. At Day 0, switch to differentiation medium, and incubate at 37°C, 5% CO2, exchanging media every 2-3 days. The GFP expression, controlled by the DMP1 promoter, is mildly observed at 5-7 days of culture under differentiation conditions, and usually peaks around day 35 of culture