Mouse HT-22 Control Cell Line

Species:: Mouse

Cell Types:: Neurons

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Product Overview

Features

Useful in studies aimed at elucidating the role of the oxidative phosphorylation in neuronal function

Cell Types

Neurons

Application Notes

Grows slow. Split 1:3 twice a week, or as needed. Change medium 1-2 times a week. Do not grow to confluence.

Species

Mouse

Properties

Size

>1x10^6 Cells

Form

Frozen

Growth Pattern

Epithelial

Culture Medium

DMEM high glucose, 10% FBS, 1 mM pyruvate, 50 ug/ml uridine

Freeze Medium

Complete growth medium supplemented with 10% DMSO

Biosafety Level

BSL1

Tissue Source

Brain/hippocampus

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Cells are sensitive to acidification of the medium.

Research Use Only

This product is intended for research use only.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

We appreciate the high quality and reliability of this product

Its consistency and reproducibility have allowed us to obtain reliable baseline data for our experiments. The cell line demonstrates stable growth and is highly responsive to our treatment conditions, which is essential for our ongoing studies.

Excellent

An excellent model for our studies

The cells exhibit stable behavior and growth, which has made it easier for us to compare experimental results with control conditions. The product's performance has been consistent, contributing significantly to our research outcomes.

Excellent

The cells are easy to culture and maintain

The cell line is robust, with minimal variability, which is critical for our comparative studies. The growth characteristics are well-documented, making it easy to integrate into our experimental protocols.

Excellent

A reliable choice for control experiments

Its reliability and consistency have allowed us to establish robust control conditions, which are essential for validating our experimental results. The cell line is easy to work with and has performed well across multiple experiments.

Excellent

It integrates well with our experimental design

We have been using the cell line for various assays, and it has proven to be a valuable tool. The cell line shows excellent stability and reproducibility, which is essential for our research on cellular mechanisms.

Q&As

What are some common experimental protocols involving the cell line?

Common experimental protocols include assessing cell viability using MTT or CCK-8 assays, evaluating oxidative stress with reactive oxygen species (ROS) assays, and analyzing neuronal apoptosis through flow cytometry or TUNEL assays. Researchers also use it for western blotting to measure protein expression levels and immunofluorescence to study cellular morphology and marker expression.

What are the key characteristics of the cell?

The mouse HT-22 control cell line is a neuronal cell line derived from the hippocampus of mice. It is characterized by its ability to form neuronal-like cells, express neuronal markers, and respond to neurotoxic stimuli. This cell line is known for its stable phenotype and can be used as a reference for studying neurotoxicity, neuroprotection, and other neuronal functions.

Are there any specific safety concerns when handling the mouse HT-22 cell?

Standard laboratory safety practices should be followed when handling the cell. While the cell line is not pathogenic, it is important to use appropriate personal protective equipment (PPE) and work within a biosafety cabinet to prevent contamination. Ensure all materials are disposed of according to biosafety protocols and practice good aseptic techniques.