Mouse Anti-TF Monoclonal Antibody (CBP1520)

Product Overview

Description

Mouse anti-transferrin antibodies can recognize the N-terminal domain of human transferrin and have been shown to block the binding of transferrin to the transferrin receptor (CD71). The clone HTF-14 cannot recognize the receptor-bound transferrin. Clone HTF-14 recognizes the conformational epitope involving key Lys-144 residues in the transferrin N-valve ring, which is also essential for the binding of the N-valve to the transferrin receptor (Mason et al. 2009)
Transferrin is a 77 kDa plasma protein synthesized by the liver that is involved in iron transport. Each transferrin molecule has two domains, and both domains can carry 2 iron ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division.

Immunogen

Purified porcine transferrin.

Species Reactivity

Human; Rabbit; Rat; Porcine

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1520

Applications

ELISA; IF; WB

Research Areas

Growth and Development

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein A.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

Transferrin

Official Name

Full Name

Transferrin

Alternative Names

Transferrin; TF

Gene ID

7018(Human); 22041(Mouse); 24825(Rat)

Uniprot ID

P02787(Human); Q921I1(Mouse); P12346(Rat)

References

1. Trebichavsky, I. et al. (1987) Monoclonal antibodies against pig transferrin. Blocking and binding activity.
2. Fuchs, O. et al. (1988) Non-transferrin donors of iron for heme synthesis in immature erythroid cells.
3. Bártek, J. et al. (1985) Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.

Publications