NeuroMab™ Mouse Anti-ITGAM Monoclonal Antibody (CBP1554)

Description

The mouse anti-Mouse Notch 1 antibody recognizes one of the four main transmembrane receptors (Notch 1-4) of the Notch signaling pathway, and the Notch 1 is activated by binding to a membrane-bound specific ligand containing the DSL domain. The Notch signaling pathway is an evolutionary conserved pathway in multicellular organisms. It is essential for cell-to-cell communication in basic developmental and physiological processes (including regulation of neurons, heart and endocrine development, stem cell hematopoiesis, thymic T cell development, and tumors). Progress and inhibition. Notch receptors are connected to physically adjacent signal-receiving cells through their specific ligands Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, through the ADAM family of metalloproteinases and gamma secretases The complex induces proteolysis of the receptor. Within the transmembrane domain, the Notch intracellular domain (NICD) is released for transport to the nucleus. Then, subsequent signal transduction occurs through the CSL-NICD-Mastermind complex cascade (standard pathway) or the NF-kappaB-NICD and CSL-NICD-Deltex complex signal cascade (non-classical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, while the non-canonical pathway promotes differentiation.

Immunogen

Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1554

Applications

FC

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1350

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

0.05 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD11b

Official Name

ITGAM

Full Name

Integrin alpha M

Alternative Names

ITGAM; CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; integrin subunit alpha M

Gene ID

3684 (Human); 16409 (Mouse); 25021 (Rat)

Uniprot ID

P11215 (Human); P05555 (Mouse)

1. Ray, W.J. et al. (1999) Evidence for a physical interaction between presenilin and Notch. 2. Huppert, S.S. et al. (2000) Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1. 3. Espinosa, L. et al. (2002) p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR. 4. Ren, M. and Cowell, J.K. (2011) Constitutive Notch pathway activation in murine ZMYM2-FGFR1-induced T-cell lymphomas associated with atypical myeloproliferative disease. 5. Kang-decker, N. et al. (2004) Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency.