Mouse Anti-RANTES Monoclonal Antibody (CBP2137)

Product Overview

Description

RANTES is a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils and binds to CCR1, CCR3, CCR4, and CCR5. It is also one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). Induced by mitogens, it is T cell and macrophage specific and belongs to the intercrine beta (chemokine CC) family.
Human RANTES Antibody (4A10F2), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified full length human recombinant RANTES protein (Swiss Prot: P13501).

Immunogen

Purified full length human recombinant RANTES protein (Swiss Prot: P13501)

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b,κ

Clone Number

CBP2137

Applications

ELISA; WB

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.

ELISA Capture: 0.5-10 μg/ml

ELISA Detection: 0.05-0.2 μg/ml
Recommended antibody pairing for sandwich immunoassay:

Western Blot: 0.5-1.0 μg/ml

Other Applications: user-optimized

Conjugation

Unconjugated

Product Properties

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification

Protein A affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Handling Advice

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CCL5

Official Name

CCL5

Full Name

chemokine (C-C motif) ligand 5

Alternative Names

3-68; C-C motif chemokine 5; SIS-delta; D17S136E; SISd; T cell-specific protein P228; EoCP; Small-inducible cytokine A5; Eosinophil chemotactic cytokine; TCP228; 4-68; RANTES; SCYA5; eoCP; T-cell-specific protein RANTES