Rat Anti-Mouse CD106 Monoclonal Antibody (CBP4346)

Product Overview

Description

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Immunogen

Mouse preadipose cell line PA6

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a, κ

Clone Number

CBP4346

Applications

FC; IHC-F; IP; ELISA

Application Notes

FCM ( ≤ 0.25 µg per million cells in 100 µL volume)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

0.2 µm filtered in PBS, pH 7.2, containing no preservative.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

VCAM1

Official Name

VCAM1

Full Name

Vascular Cell Adhesion Molecule 1

Alternative Names

Vascular Cell Adhesion Molecule 1; CD106 Antigen; INCAM-100; Vascular Cell Adhesion Protein 1; V-CAM 1; VCAM-1; CD106;

Gene ID

7412(Human); 22329(Mouse); 25361(Rat)

Uniprot ID

P19320(Human); P29533(Mouse); P29534(Rat)

References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Publications