Rat huntingtin (Htt) (NM_024357) ORF clone, Untagged

Description

This is a rat HTT untagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

HTT

Species

Rat

Insert Size (bp)

9363 bp

Tag

Tag Free

Type

Expression Vector/ Viral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Kanamycin (25 ug/mL)

Cell Selection

Neomycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGCAACCCTGGAAAAACTGATGAAGGCTTTCGAGTCGCTCAAGTCGTTCCAGCAGCAACAGCAGCAGCAGCAGCCGCCGCCGCAGGCGCCGCCACCACCGCCGCCGCCGCCGCCTCAACCCCCTCAGCCGCCGCCTCAGGGGCAGCCGCCGCCACCACCGCCGCTGCCAGGTCCGGCCGAGGAGCCGCTGCACCGACCAAAGAAGGAACTCTCAGCCACCAAGAAGGACCGTGTGAATCACTGTCTAACAATATGTGAAAACATTGTGGCACAGTCTCTCAGAAATTCTCCAGAATTTCAGAAACTCTTGGGCATTGCTATGGAACTGTTTCTGCTGTGCAGCGACGATGCGGAGTCAGACGTCAGAATGGTGGCTGATGAGTGCCTCAACAAAGTCATCAAAGCTTTGATGGACTCTAATCTTCCAAGGCTACAGTTAGAACTCTATAAGGAAATTAAAAAGAATGGTGCTCCTCGAAGTTTGCGTGCAGCTCTGTGGAGGTTTGCTGAGCTGGCTCACCTGGTTCGACCTCAGAAGTGCAGGCCTTATCTGGTGAATCTTCTTCCATGTTTGACCCGAACAAGCAAACGACCGGAGGAGTCAGTTCAGGAGACTTTGGCTGCAGCTGTTCCTAAAATTATGGCCTCTTTTGGCAATTTCGCGAATGACAATGAAATTAAGGTTCTATTGAAAGCTTTCATAGCAAATCTGAAGTCAAGCTCTCCCACTGTGCGGCGGACAGCAGCTGGGTCAGCAGTGAGTATCTGCCAGCACTCTAGGAGGACACAGTACTTCTACAACTGGCTCCTGAATGTGCTCCTAGGTTTGCTGGTTCCCATGGAGGAAGACCACCCCACTCTCCTGATCCTTGGTGTGTTGCTCACACTGAGGTGTCTAGTGCCCTTGCTCCAGCAGCAGGTCAAGGACACAAGTCTAAAGGGCAGCTTTGGGGTAACACGGAAAGAAATGGAAGTCTCTCCTTCTGCAGAGCAGCTTGTCCAGGTTTATGAACTGACTTTGCATCACACACAGCACCAAGACCATAATGTGGTGACAGGGGCATTGGAGCTCCTGCAGCAGCTCTTCCGTACCCCTCCACCTGAGCTGCTGCAAGCACTGACCACACCAGGAGGGCTCGGGCAGCTCACTCTGGTTCGAGAGGAAGCCGGGGGCCGAGGCCGCAGCGGGAGTATCGTGGAGCTTTTAGCTGGAGGGGGTTCCTCATGCAGCCCTGTTCTCTCAAGAAAGCAAAAAGGCAAAGTGCTCTTAGGAGAGGAAGAAGCCTTGGAGGATGACTCGGAGTCCAGGTCAGATGTCAGCAGCTCAGCCTTTGCAGCCTCTGTGAAGAGTGAGATTGGTGGAGAGCTCGCTGCTTCTTCTTCGGGTGTCTCCACTCCCGGTTCTGTAGGTCACGACATCATCACTGAGCAGCCTCGATCCCAGCACACACTTCAAGCAGACTCTGTGGATTTGTCAGGCTGTGACTTGACCAGTGCTGCTACTGATGGAGATGAGGAAGACATCTTGAGCCACAGCTCCAGCCAGTTCAGTGCTGTTCCATCCGACCCTGCCATGGACCTGAATGATGGGACCCAGGCCTCCTCACCCATCAGTGACAGTTCTCAGACCACCACTGAAGGACCTGATTCAGCTGTGACTCCTTCTGACAGTTCTGAAATTGTCTTAGATGGTGCTGACAGCCAGTATTTAGGCGTGCAGATAGGACAGCCACAGGAGGAAGACGAGGAGGAAGCTGCAGGTGTTCTTTCTGGTGAAGTCTCAGACGTTTTCAGAAACTCTTCTCTGGCCCTTCAGCAGGCACACTTGTTGGAAAGAATGGGTCATAGCCGGCAGCCTTCTGACAGCAGTGTTGATAAGTTTGTTTCAAAAGATGAGGTTGCTGAAGCTGGGGACCCAGAAAGCAAGCCTTGCCGAATCAAAGGTGACATAGGACAGCCTAATGATGATGATTCTGCTCCTCTGGTACATTGTGTCCGTCTTTTATCCGCTTCCTTTTTGTTAACTGGCGAAAAGAAAGCACTGGTTCCAGACAGAGATGTGAGAGTCAGTGTGAAGGCCCTGGCCCTCAGCTGTATTGGTGCAGCTGTGGCCCTTCATCCAGAGTCGTTCTTCAGCAAACTCTACAAAGTACCTCTCAGTACCATGGAAAGTACTGAGGAACAGTATGTCTCTGACATCCTGAACTACATCGATCATGGAGACCCTCAGGTGCGAGGAGCTACTGCCATTCTCTGTGGGACCCTTGTCTACTCCATCCTCAGCAGGTCCCGTCTCCGTGTTGGTGACTGGCTGGGCACCATCAGGGCCCTGACAGGAAATACATTTTCTCTGGTGGACTGCATTCCTTTACTGCAGAAAACTTTGAAGGATGAATCTTCTGTTACTTGCAAGTTGGCTTGTACAGCTGTGAGGCACTGTGTCCTGAGTCTTTGCAGCAGCAGCTACAGTGACTTGGGATTACAACTGCTTATTGACATGCTGCCTCTGAAGAACAGCTCCTACTGGCTGGTGAGGACTGAACTGCTGGAAACTCTTGCAGAGATTGATTTCAGGCTGGTGAGTTTTTTGGAGGCAAAAGCAGAAAGTTTACACCGAGGGGCTCATCATTATACAGGGTTTCTAAAACTACAAGAACGAGTACTCAATAATGTGGTCATTTATTTGCTTGGAGATGAAGACCCCAGGGTTCGACATGTTGCTGCGACGACATTGACAAGACTTGTCCCAAAGCTGTTTTATAAGTGTGACCAAGGACAGGCTGACCCAGTCGTGGCTGTAGCAAGAGATCAAAGTAGTGTTTACCTGAAGCTCCTCATGCATGAGACCCAGCCACCATCCCACTTCTCCGTCAGCACCATAACCAGAATCTATAGAGGCTACAGCTTACTACCAAGTGTAACAGATGTCACCATGGAAAACAACCTCTCAAGAGTCGTTGCCGCAGTTTCTCATGAACTCATTACGTCAACTACACGGGCACTCACATTTGGGTGCTGTGAAGCCTTGTGTGTTCTTTCAGCCGCCTTTCCAGTTTGCACTTGGAGTCTAGGATGGCACTGTGGAGTGCCCCCACTGAGTGCCTCTGATGAGTCCAGGAAGAGCTGCACTGTTGGGATGGCCTCCATGATTCTCACCTTGCTTTCATCAGCTTGGTTCCCACTGGATCTCTCAGCCCATCAGGATGCCTTGATTTTGGCTGGAAACTTGCTAGCAGCGAGTGCCCCCAAGTCTCTGAGAAGCTCATGGGCCTCGGAAGAAGAAGGCAGCTCAGCAGCCACCAGACAGGAGGAGATCTGGCCTGCCCTGGGGGATCGGACTCTGGTGCCCATGGTGGAGCAGCTTTTCTCCCACCTGCTGAAGGTGATCAATATCTGTGCTCATGTCTTGGATGACGTGACTCCTGGACCAGCAATCAAGGCAGCTTTGCCTTCTCTCACAAACCCCCCTTCTCTAAGTCCTATTCGACGGAAAGGGAAGGAGAAAGAGCCCGGAGAACAAACATCCACTCCGATGAGTCCCAAGAAAGGTGGAGAGGCCAGTACAGCCTCTCGACAGTCAGACACCTCAGGACCTGTCACAGCGAGTAAATCATCTTCACTTGGGAGTTTCTACCATCTCCCTTCCTACCTCAGACTGCATGATGTCCTGAAAGCCACTCACGCCAACTATAAGGTCACCTTAGATCTTCAGAACAGCACTGAAAAGTTTGGGGGGTTCCTGCGCTCTGCCTTGGACGTCCTTTCTCAGATTCTAGAGCTGGCGACACTGCAGGACATTGGAAAGTGTGTTGAAGAGGTCCTTGGATACTTGAAATCCTGCTTTAGTCGAGAACCAATGATGGCGACTGTCTGTGTTCAGCAGCTATTGAAGACTCTCTTTGGGACAAACTTAGCCTCACAGTTTGATGGCTTATCTTCCAACCCCAGCAAGTCTCAGTGCCGAGCACAGCGCCTTGGCTCTTCCAGTGTGAGGCCCGGCTTATATCACTACTGCTTCATGGCACCATACACGCACTTCACGCAGGCTTTGGCTGATGCCAGCCTGAGGAACATGGTACAGGCGGACCAGGAGCACGATGCCTCAGGGTGGTTTGATGTACTCCAGAAAGTGTCTGCTCAGTTGAAGACGAACCTTACAAGTGTCACAAAGAACCGTGCAGATAAGAACGCTATTCATAACCACATTAGGTTATTTGAGCCTCTTGTTATAAAAGCATTGAAGCAGTACACCACGACAACATCAGTACAACTGCAGAAGCAGGTTTTGGATTTGCTGGCACAGCTGGTTCAGCTACGGGTCAATTACTGTCTACTGGATTCAGATCAGGTGTTCATCGGGTTTGTGCTGAAGCAGTTTGAGTACATTGAAGTGGGCCAGTTCAGGGAATCAGAGGCAATTATTCCAAATATATTTTTCTTCCTGGTACTATTATCTTATGAGCGCTACCATTCAAAACAGATCATTGGAATTCCTAAAATCATCCAGCTGTGTGATGGCATCATGGCCAGTGGAAGGAAGGCTGTCACACATGCTATTCCTGCGCTGCAGCCCATTGTCCATGACCTCTTTGTGTTAAGAGGAACAAATAAAGCTGATGCAGGGAAAGAGCTTGAAACCCAGAAGGAGGTGGTGGTCTCAATGCTGTTACGACTCATCCAGTACCATCAGGTGCTAGAGATGTTCATCCTCGTCCTGCAGCAGTGCCACAAAGAGAATGAGGACAAGTGGAAACGGCTCTCTCGGCAGGTCGCAGACATCATCCTGCCCATGTTAGCCAAGCAGCAGATGCATATTGACTCTCATGAAGCCCTTGGAGTATTAAATACCTTGTTTGAGATTTTGGCTCCTTCCTCCCTACGTCCTGTGGACATGCTTTTGCGGAGTATGTTCATCACTCCAAGCACAATGGCATCTGTAAGCACTGTGCAGCTGTGGATATCTGGAATCCTAGCCATTCTGAGGGTTCTCATTTCCCAGTCAACCGAAGACATTGTTCTTTCTCGTATTCAGGAGCTCTCCTTCTCTCCATATTTAATTTCCTGTCCAGTAATTAACAGGTTAAGGGATGGAGACAGTAATCCAACACTAGGAGAACGCAGTGAAGGGAAACAAGTAAAGAATTTGCCAGAAGATACATTCTCAAGGTTTCTCTTACAGCTGGTTGGTATTCTTCTGGAAGACATTGTTACAAAACAGCTCAAAGTGGACATGAGTGAACAGCAGCATACATTCTATTGCCAAGAGCTCGGCACACTGCTCATGTGTCTGATCCACATATTCAAATCTGGAATGTTCCGGAGAATCACAGCCGCTGCCACTAGACTCTTCACCAGTGATGGCTGTGAAGGCAGCTTCTATACTCTAGATAGCCTGAATGCACGGGTGCGAGCCATGGTGCCCACACACCCAGCTCTGGTACTGCTCTGGTGTCAGATCCTACTGCTCATCAACCACACTGACCACCGATGGTGGGCCGAGGTGCAGCAGACGCCCAAGAGACACAGTCTGTCCTGCACGAAGTCACTAAACCCCCAGATATCTGCTGAAGAGGATTCTGGCTCAGCAGCTCAGCTTGGAATGTGCAATAGAGAAATAGTACGAAGAGGGGCCCTTATTCTCTTCTGTGATTATGTCTGTCAGAATCTCCATGACTCAGAACACTTAACATGGCTCATTGTGAATCACATTCAAGATCTGATCAGCTTGTCCCACGAGCCTCCAGTTCAAGACTTTATTAGTGCCATTCATCGTAATTCTGCAGCTAGTGGTCTTTTTATCCAGGCAATTCAGTCTCGCTGTGAAAATCTTTCAACTCCAACCACTCTGAAGAAAACACTTCAGTGCTTGGAAGGCATCCATCTCAGCCAGTCTGGTGCTGTGCTCACACTGTATGTGGACAGGCTACTGGGCACCCCTTTCCGTGCGCTGGCTCGCATGGTCGACACCCTGGCCTGTCGCCGAGTAGAAATGCTTTTGGCTGCAAATTTACAGAGCAGCATGGCCCAGTTGCCAGAGGAGGAACTGAACAGAATCCAGGAACACCTCCAGAACACTGGGCTTGCACAAAGACACCAAAGGCTCTATTCACTGCTGGACAGATTCCGACTCTCTACTGTGCAGGACTCACTTAGCCCCTTGCCCCCAGTCACTTCCCACCCTCTGGATGGGGATGGGCACACATCCCTGGAAACAGTGAATCCGGACAAAGACTGGTACCTCCAGCTTGTCAGATCCCAGTGTTGGACCAGGTCAGATTCTGCACTGCTGGAAGGTGCAGAGCTGGTGAACCGTATCCCTGCTGAAGATATGAGTGACTTCATGATGAGCTCGGAGTTCAACCTAAGCCTTTTGGCTCCCTGCTTAAGCCTTGGCATGAGCGAGATTGCTAATGGCCAAAAGAGTCCCCTTTTTGAAGCGGCTCGTAGGGTGACTCTGGACCGGGTGACCAATGTGGTTCAGCAGCTGCCTGCAGTCCATCAAGTCTTCCAGCCTTTCCTGCCTACAGAACCCACAGCCTACTGGAGCAAGCTGAATGATCTCTTTGGTGATACCACATCATACCAGTCTCTGACCACACTTGCCCGTGCCCTGGCACAGTACCTGGTGGTGCTCTCCAAAGTGCCTGCTCCTTTGCACCTTCCTCCTGAGAAGGAGGGGCACACGGTGAAGTTTGTGGTAATGACACTTGAGGCCCTGTCATGGCATTTGATCCATGAGCAGATCCCACTGAGTCTGGACCTCCAAGCCGGCCTAGACTGCTGCTGCCTGGCACTGCAGGTGCCTGGCCTCTGGGGGGTGCTGTCCTCCCCAGAGTACGTGACTCATACTTGCTCCCTTATCCACTGTGTGCGATTCATCCTGGAAGCCATTGCAGTACAACCTGGAGACCAACTTCTTGGTCCGGAAAGCAGGTCACATACTCCAAGGGCTGTCAGAAAGGAGGAAGTAGACTCAGATATACAAAACCTCAGTCACATCACTTCGGCCTGCGAGATGGTGGCAGACATGGTGGAATCCCTGCAGTCGGTGCTGGCCCTGGGCCACAAGAGGAACAGCACCCTACCTTCATTTCTCACAGCTGTGCTGAAGAACATTGTTGTCAGTCTGGCCCGCCTCCCCCTCGTTAACAGCTATACTCGTGTGCCTCCTCTGGTATGGAAACTCGGGTGGTCACCCAAGCCTGGAGGGGATTTCGGCACAGTGTTTCCTGAGATCCCTGTAGAGTTCCTCCAGGAGAAGGAGGTCCTCAAGGAGTTCATCTACCGCATCAACACCCTAGGGTGGACCAGTCGTACTCAATTCGAAGAAACTTGGGCCACCCTCCTTGGTGTCCTGGTGACTCAGCCCTTGGTGATGGAACAGGAAGAGAGCCCACCAGAGGAAGACACCGAAAGGACCCAGATCCACGTCCTGGCTGTACAGGCCATCACCTCTCTAGTGCTCAGCGCAATGGCTGTGCCTGTGGCTGGCAATCCAGCTGTAAGCTGCTTGGAGCAACAGCCCCGGAACAAGCCACTGAAGGCTCTCGATACCAGATTTGGAAGAAAGTTGAGCATGATCAGAGGGATTGTAGAACAAGAAATCCAAGAGATGGTTTCCCAAAGAGAGAATACTGCCACTCATCATTCTCACCAGGCATGGGATCCTGTCCCTTCTCTGTTACCAGCTACTACAGGTGCTCTTATCAGCCATGACAAGCTGCTGCTGCAGATCAACTCAGAGCGGGAGCCAGGCAACATGAGCTACAAGCTGGGCCAGGTGTCCATACACTCCGTGTGGCTGGGGAACAACATCACACCCCTGAGAGAGGAGGAATGGGATGAGGAGGAGGAGGAAGAAGCGGATGCCCCTGCGCCAACATCACCACCTGTGTCTCCAGTCAATTCCAGAAAACACCGTGCTGGGGTTGATATTCACTCCTGTTCGCAGTTTCTGCTTGAATTATACAGCCGTTGGATCCTGCCATCCAGTGCAGCCAGAAGGACCCCTGTCATCCTGATCAGTGAAGTGGTTCGATCTCTTCTTGTGGTGTCAGACTTATTCACTGAACGTACCCAGTTTGAAATGATGTATCTGACGCTGACAGAACTACGGAGAGTGCACCCTTCAGAAGATGAGATCCTCATTCAATACCTGGTGCCTGCCACCTGTAAGGCAGCTGCTGTTCTTGGAATGGACAAAACTGTGGCAGAGCCGGTCAGCCGCCTACTGGAGAGCACACTCAGGAGCACCCACCTGCCCAGCCAGATCGGAGCCCTGCATGGCATCCTCTATGTGTTGGAGTGTGACCTCTTGGATGACACTGTAAAGCAGCTCATTCCAGTTGTTAGTGACTATCTGCTGTCCAACCTCAAAGGAATAGCCCACTGCGTGAACATTCACAGCCAGCAGCATGTGCTGGTGATGTGTGCCACTGCATTCTACCTGATGGAAAACTACCCTCTGGATGTGGGGCCAGAATTCTCAGCATCTGTGATACAGATGTGTGGAGTAATGCTGTCTGGAAGTGAGGAGTCCACCCCCTCCATCATTTACCACTGTGCCCTCCGGGGTCTGGAACGGCTCCTGCTGTCTGAGCAGCTCTCTCGGCTAGACACGGAGTCCTTGGTCAAGCTAAGTGTGGACAGAGTGAATGTACAAAGCCCACACAGGGCCATGGCAGCCCTAGGCCTGATGCTTACCTGCATGTACACAGGAAAGGAAAAAGCCAGTCCAGGCAGAGCTTCTGACCCCAGCCCTGCTACCCCTGACAGCGAGTCTGTGATTGTAGCTATGGAGCGAGTGTCTGTGCTCTTTGACAGGATCCGCAAGGGATTTCCCTGTGAAGCCAGGGTCGTGGCAAGGATCCTGCCTCAGTTTCTAGATGACTTCTTTCCACCTCAAGATGTCATGAACAAAGTCATTGGAGAGTTCCTGTCCAACCAGCAGCCATACCCACAGTTCATGGCCACTGTAGTATACAAGGTTTTTCAGACTCTGCACAGTGCTGGGCAGTCATCCATGGTCCGGGACTGGGTTATGCTGTCTCTGTCCAACTTCACACAAAGAACTCCAGTTGCCATGGCCATGTGGAGCCTCTCCTGCTTCCTTGTCAGTGCATCTACCAGCCCATGGGTTTCTGCAATCCTTCCACACGTCATCAGCAGGATGGGCAAACTGGAGCAGGTGGATGTGAACCTTTTCTGCCTGGTTGCCACAGACTTCTACAGACACCAGATAGAGGAGGAATTCGACCGCAGGGCTTTCCAGTCTGTGTTTGAGGTGGTGGCAGCACCAGGAAGTCCATACCACAGGCTGCTTGCTTGTTTGCAAAATGTTCACAAGGTCACCGCCTGCTGAACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

HTT