Creative Biolabs

Human APOE Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP513]

Human APOE knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HEK 293T

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Description
This is a HEK293T cell transfected lysate in which Human APOE has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HEK 293T
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
Tissue Source
Kidney
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Apo E
Official Name
APOE
Full Name
Apolipoprotein E
Alternative Names
APOE; AD2; APO-E; LDLCQ5; LPG; apolipoprotein E; ApoE4
Gene ID
348 (Human); 11816 (Mouse)
Uniprot ID
P02649 (Human); P08226 (Mouse)
For Research Use Only. Not For Clinical Use.
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