Creative Biolabs

Human FTL Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP564]

Human FTL knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

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Description
This is a HeLa cell transfected lysate in which Human FTL has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Ferritin Light Chain
Official Name
FTL
Full Name
Ferritin Light Chain
Alternative Names
Ferritin Light Chain; Ferritin L Subunit; Ferritin Light Polypeptide-Like 3; Ferritin; Light Polypeptide; Ferritin L-Chain; Neurodegeneration With Brain Iron Accumulation 3; L Apoferritin; NBIA3; LFTD;
Gene ID
2512(Human); 14325(Mouse); 29292(Rat)
Uniprot ID
P02792(Human); P29391(Mouse); P19132(Rat)
For Research Use Only. Not For Clinical Use.
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