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Creative Biolabs

Human INA Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP457]

Human INA knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human INA has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 179 bp deletion in exon1 and 1 bp insertion in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Cell Adhension Protein

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

Alpha Internexin

Official Name

INA

Full Name

alpha Internexin

Alternative Names

NEF5

Gene ID

9118 (Human); 226180 (Mouse); 24503 (Rat)

Uniprot ID

Q16352 (Human); P46660 (Mouse); P23565 (Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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