Creative Biolabs

Human INA Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP457]

Human INA knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

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Description
This is a HeLa cell transfected lysate in which Human INA has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 179 bp deletion in exon1 and 1 bp insertion in exon1.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Cell Adhension Protein
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Alpha Internexin
Official Name
INA
Full Name
alpha Internexin
Alternative Names
NEF5
Gene ID
9118 (Human); 226180 (Mouse); 24503 (Rat)
Uniprot ID
Q16352 (Human); P46660 (Mouse); P23565 (Rat)
For Research Use Only. Not For Clinical Use.
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