Human mammalian target of rapamyein, mTOR ELISA Kit

[CAT#: NRZP-0722-ZP252]

For the quantitative determination of human mammalian target of rapamyein (mTOR) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human mTOR.

Species Reactivity

Human

Marker

Mammalian target of rapamyein,mTOR

Properites

Sensitivity

In most cases, the sensitivity is below 0.39pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

1.56 pg/ml-100 pg/ml

Max Emission

450 nm

Target Details

Target

mTOR

Official Name

mTOR

Full Name

mechanistic target of rapamycin kinase

Alternative Names

SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Gene ID

2475(Human)

Uniprot ID

P42345(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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