Creative Biolabs

Human PRKN Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP504]

Human PRKN knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Bulk Quote
Description
This is a HeLa cell transfected lysate in which Human PRKN has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon3 and 1 bp deletion in exon3.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Parkinson's Disease Signaling
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Parkin
Official Name
PRKN
Full Name
Parkin
Alternative Names
PRKN; parkin RBR E3 ubiquitin protein ligase; AR-JP; LPRS2; PDJ; PARK2
Gene ID
5071(Human); 50873(Mouse); 56816(Rat)
Uniprot ID
O60260(Human); Q9WVS6(Mouse); Q9JK66(Rat)
For Research Use Only. Not For Clinical Use.
Send Inquiry Send Inquiry
Inquiry Basket
compare

Go to compare