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Creative Biolabs

Mouse Kupffer Cell Line (ImKC), Immortalized

[CAT#: NCL-21P6-176] Review(5) Q&As(3)

Species:
Mouse
Applications:
Cell Culture
Cell Types:
Other Cells

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Product Overview

Description

The Mouse Kupffer Cell Line (ImKC) is an immortalized cell line derived from mouse Kupffer cells, which are the liver macrophages responsible for various immune functions in the liver. The cell line from Creative Biolabs is generated by transformating with SV40 and characterized with F4/80.

Cell Types

Other Cells

Applications

Cell Culture

Species

Mouse
Properties

Form

Cryopreserved

Biosafety Level

1

Storage

Liquid Nitrogen

Research Use Only

For research use only
Publications

Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
I’ve been using the ImKC cell line for my liver research
The cells exhibit robust viability and have been incredibly responsive to cytokine treatments. The immortalization does not seem to affect their primary characteristics, making them ideal for long-term experiments
user
Excellent
Their response to LPS stimulation has been particularly noteworthy
We’ve used it for several inflammation studies and found the cells to be consistent and reliable. The immortalized nature of the cells means we can carry out extended studies without worrying about variability.
user
Excellent
Excellent product
Our lab focuses on liver fibrosis, and the ImKC line has been crucial for our in vitro studies. The cells show expected phagocytic activity and respond well to fibrogenic stimuli. We've also successfully used them in co-culture systems with hepatocytes, observing significant interactions that mirror in vivo conditions.
user
Excellent
This cell line is a fantastic tool
As part of our research into liver diseases, we’ve utilized the ImKC line extensively. The cells are easy to culture and maintain their characteristics over multiple passages. We’ve performed a variety of assays, including cytokine release and phagocytosis, and the results have been consistent and reliable.
user
Excellent
Their ability to maintain functionality over extended periods is a major advantage
The cell has made a significant impact on our research in hepatic immunology. We’ve used these cells to study the effects of various drugs on liver macrophages. The cells are highly responsive to treatments and provide reproducible results.
Q&As
What are the culture conditions required for maintaining the ImKC?
The ImKC should be cultured in a suitable growth medium, typically consisting of DMEM supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, and 1% L-glutamine. The cells should be maintained at 37°C in a humidified atmosphere with 5% CO2. It is crucial to follow these conditions to ensure optimal cell growth and functionality.
What are the advantages of using the ImKC over primary Kupffer cells?
The ImKC offers several advantages over primary Kupffer cells. Immortalized cells provide a consistent and renewable source, eliminating the variability and limited availability associated with primary cells. They offer greater reproducibility in experiments and can be cultured for extended periods without significant changes in phenotype. This makes them ideal for long-term studies and high-throughput screening applications.
How do I troubleshoot if the ImKC shows unexpected behavior in my experiments?
If the ImKC exhibits unexpected behavior, several troubleshooting steps can be taken. First, verify that the culture conditions, including medium composition, temperature, and CO2 levels, are optimal. Check for contamination by mycoplasma or other microorganisms. Ensure that the cells have been authenticated and are not cross-contaminated with other cell lines. Reviewing the experimental protocols and comparing them with established methods can also help identify any deviations. If issues persist, contacting our technical support team for assistance is recommended.
For Research Use Only. Not For Clinical Use.
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