Product

iNeu™ Human Mesenchymal Stem Cells

[CAT#: NCL-2103-P69]

Species:: Human

Cell Types:: Mesenchymal Stem Cell

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Product Overview

Description

Mesenchymal stem cells ("MSCs") are non-hematopoietic, pluripotent stromal stem cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, muscle cells and adipocytes. iNeu™ is a mesenchymal stem cell derived from human iPSC. In addition to Oct4 and Nanog, iNeu™ also expresses cell surface markers such as CD73, CD90, CD166 and CD105. This expression profile predicts the long-term proliferation ability of cultured human MSCs. MSCs have immunomodulatory functions, secrete cytokines and immune receptors, can regulate the microenvironment in host tissues, and they have multi-lineage potential for multiple cell types. MSC can be used for a variety of screening applications, from screening biological agents and small molecules to siRNA. Since MSC has the ability to self-renew (= grow without differentiation), it can be easily expanded for large-scale assays.

Cell Types

Mesenchymal Stem Cell

Application Notes

Applications:

Developmental studies
Biomarker discoveries and validations
Lineage differentiation studies
Large-scale compound screenings
In vitro assays using MSCs (e.g., cytokine release assays)

Species

Human

Properties

Size

~0.6x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.