
iNeu™ Human Neural Stem Cells (Male), Source Cord Blood CD34+ Cells, Validated
- Species:
- Human
- Cell Types:
- Neural Stem Cells
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Handling Advice
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
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Immunocytochemistry/ Immunofluorescence of Human iPSC-Derived Glutamatergic Neurons
Immunocytochemistry of Glutamatergic Neurons at day 9 post plating shows homogeneous expression of pan neuronal marker MAP2 and a dense neuronal network.

Immunocytochemistry/ Immunofluorescence of Human iPSC-Derived Glutamatergic Neurons
Immunofluorescent staining on post-revival day 11 demonstrates homogenous expression of pan-neuronal proteins (MAP2 and TUBB3) and glutamatergic neuron-specific transporters (VGLUT1 and VGLUT2). Cells exhibit neurite outgrowth.

Functional Studies of Human iPSC-Derived Glutamatergic Neurons
Glutamatergic Neurons after revival over the course of the first 11 days of culture. Day 1 to 11 post-thawing; 400X magnification; scale bar: 100µm

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