M1 Muscarinic Acetylcholine Receptor Assay Cell Line
[CAT#: NCL2110P280]
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: M1 (CHRM1)/U2OS
Official Full Name: Muscarinic acetylcholine receptor M1
DNA Accession Number: GenBank: BC007740
Host Cell: U2OS
Format: cryopreserved vials
Resistance: G418
Storage: Liquid Nitrogen
Official Full Name: Muscarinic acetylcholine receptor M1
DNA Accession Number: GenBank: BC007740
Host Cell: U2OS
Format: cryopreserved vials
Resistance: G418
Storage: Liquid Nitrogen
Features
Each vial or HiTector M1 contains U2OS cells stably expressing unlabeled human muscarinic acetylcholine receptor M1. The Creative Biolabs M1 cell line is designed to test compounds or analyze their ability to modulate the muscarinic acetylcholine receptor M1. When the agonist binds to M1, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (calcium). This cell line has been validated by measuring the increase in calcium in the cytosol. The high reproducibility of this assay allows monitoring of the M1 activation process in high-throughput screening.
Cell Types
Other Cells
Research Areas
GPCR
Assay
M1 Muscarinic Acetylcholine Receptor Assay
Assay Description
The M1 Muscarinic Acetylcholine Receptor Assay (CHRM1) from Creative Biolabs allows compounds to be tested or analyzed for their ability to modulate CHRM1. When the ligand binds to CHRM1, it activates the G protein; this in turn triggers calcium Mediated cell response. The combination of agonist and CHRM1 induces intracellular calcium mobilization and PKC activation through PLC-IP3 and DAG pathways, respectively.
The M1 muscarinic acetylcholine receptor test involves measuring the concentration of calcium in the cytoplasm. The high reproducibility of this assay allows monitoring the activation of CHRM1 receptors in living cells.
The M1 muscarinic acetylcholine receptor test involves measuring the concentration of calcium in the cytoplasm. The high reproducibility of this assay allows monitoring the activation of CHRM1 receptors in living cells.
Assay Characteristics
Readout: Calcium Flux
Agonist: Oxotremorine
EC50 Agonist: 4.08 x 106-8 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Agonist: Oxotremorine
EC50 Agonist: 4.08 x 106-8 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
M1 Muscarinic Receptor
Official Name
CHRM1
Full Name
M1 Muscarinic Acetylcholine Receptor
Alternative Names
CHRM1; HM1; M1; M1R; cholinergic receptor muscarinic 1
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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