M1 Muscarinic Acetylcholine Receptor Assay Cell Line

[CAT#: NCL2110P280]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: M1 (CHRM1)/U2OS
Official Full Name: Muscarinic acetylcholine receptor M1
DNA Accession Number: GenBank: BC007740
Host Cell: U2OS
Format: cryopreserved vials
Resistance: G418
Storage: Liquid Nitrogen

Features

Each vial or HiTector M1 contains U2OS cells stably expressing unlabeled human muscarinic acetylcholine receptor M1. The Creative Biolabs M1 cell line is designed to test compounds or analyze their ability to modulate the muscarinic acetylcholine receptor M1. When the agonist binds to M1, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (calcium). This cell line has been validated by measuring the increase in calcium in the cytosol. The high reproducibility of this assay allows monitoring of the M1 activation process in high-throughput screening.

Cell Types

Other Cells

Research Areas

GPCR

Assay

M1 Muscarinic Acetylcholine Receptor Assay

Assay Description

The M1 Muscarinic Acetylcholine Receptor Assay (CHRM1) from Creative Biolabs allows compounds to be tested or analyzed for their ability to modulate CHRM1. When the ligand binds to CHRM1, it activates the G protein; this in turn triggers calcium Mediated cell response. The combination of agonist and CHRM1 induces intracellular calcium mobilization and PKC activation through PLC-IP3 and DAG pathways, respectively.

The M1 muscarinic acetylcholine receptor test involves measuring the concentration of calcium in the cytoplasm. The high reproducibility of this assay allows monitoring the activation of CHRM1 receptors in living cells.

Assay Characteristics

Readout: Calcium Flux

Agonist: Oxotremorine

EC50 Agonist: 4.08 x 10⁶-8 M for Calcium Flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.