Mouse Anti-FUS Monoclonal Antibody (CBP2625)

Description

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases.

Expression Host

Mammalian cells

Immunogen

FUS/TLS fusion protein Ag2150

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2625

Applications

FC; IF; IHC; IP; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; IP : 0.5-4.0 ug

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

FUS

Official Name

FUS

Full Name

Fusion (involved in t(12; 16) in malignant liposarcoma)

Alternative Names

75 kDa DNA pairing protein; FUS; FUS CHOP; FUS1; FUS-CHOP; hnRNP P2; Oncogene FUS; Oncogene TLS

Gene ID

2521(Human); 233908(Mouse); 317385(Rat)

Uniprot ID

P35637(Human); P56959(Mouse)

1. Lee, E. B., Russ, J., Jung, H., Elman, L. B., Chahine, L. M., Kremens, D.,... & McCluskey, L. F. (2013). Topography of FUS pathology distinguishes late-onset BIBD from aFTLD-U. Acta neuropathologica communications, 1(1), 9. 2. Collins, M., Li, Y., & Bowser, R. (2020). RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases. Acta neuropathologica communications, 8(1), 1-25. 3. McGurk, L., Lee, V. M., Trojanowksi, J. Q., Van Deerlin, V. M., Lee, E. B., & Bonini, N. M. (2014). Poly-A binding protein-1 localization to a subset of TDP-43 inclusions in amyotrophic lateral sclerosis occurs more frequently in patients harboring an expansion in C9orf72. Journal of Neuropathology & Experimental Neurology, 73(9), 837-845.