Rat RG2 [D74] Cells

[CAT#: NCB2108FY0052]

Animal cells

Species:: Rat

Applications:: 3D Cell Culture

Cell Types:: Tumor Cells

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Product Overview

Description

Incubate the culture at 37°C in a suitable incubator.

Cell Types

Tumor Cells

Applications

3D Cell Culture

Application Notes

This cell line may be used for both in vitro and in vivo studies of a rat brain tumor. It grows well in cell culture and provides a simple, reproducible glioma model when inoculated into the brains of syngeneic rats.

Relevant Diseases

Glioma

Research Areas

Neural Cell

Species

Rat

Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Glial

Culture Medium

The base medium for this cell line is Dulbecco's modified Eagle's medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Freeze Medium

Complete growth medium supplemented with 5% (v/v) DMSO

Tissue Source

Brain

Age

20 days gestation

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Research Use Only

For research use only

Donor Information

Donor Health

Tumor

Additional Donor Information

RG2 was submitted to the American Type Culture Collection at the 3rd generation in March 1999 and was derived from tumor cultures.
This tumor was produced by Drs. A. Koestner and W. Wechsler at Ohio State University in 1971.

Publications