Creative Biolabs

Human HTT Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP555]

Human HTT knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

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Description
This is a HeLa cell transfected lysate in which Human HTT has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon21.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Huntington's Disease Signaling
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Huntingtin
Official Name
HTT
Full Name
huntingtin gene
Alternative Names
HTT; HD; IT15; huntingtin; LOMARS
Gene ID
3064(Human); 15194(Mouse); 29424(Rat)
Uniprot ID
P42858(Human); P42859(Mouse); P51111(Rat)
For Research Use Only. Not For Clinical Use.
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