SLC6A3/DAT1 293T Cell Transient Overexpression Lysate

[CAT#: NRZP-0822-ZP3436] Review(5) Q&As(3)

Cell Types:: Other Cells

Cell Subtypes:: Overexpression Stable Cell Lines

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Product Overview

Cell Types

Other Cells

Cell Subtypes

Overexpression Stable Cell Lines

Properties

Shipping

Dry ice

Storage

Store in liquid nitrogen

Research Use Only

For Research Use Only

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Case Studies

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

An excellent addition to our Western blot experiments

The lysate provided clear and reproducible bands for the DAT1 protein, and the overexpression system was very effective. We observed strong and specific signals with minimal background noise, which is crucial for our quantitative analysis.

Excellent

Highly recommended for similar applications

We used this lysate to investigate dopamine transporter expression levels, and it performed admirably. The overexpression in 293T cells gave us high yield and quality of the protein. The Western blot results were consistent and allowed us to distinguish between different isoforms effectively.

Excellent

The SLC6A3/DAT1 lysate was very reliable for our studies

We achieved clear bands with good intensity and minimal degradation. The lysate was easy to handle, and the results were reproducible across multiple experiments. This product significantly advanced our research.

Excellent

This lysate was ideal for our Western blot assays

The overexpression in 293T cells provided ample protein for our experiments, and the DAT1 signal was strong and specific. We encountered no issues with background or non-specific binding, which is a testament to the product’s quality.

Excellent

The lysate was well-prepared and easy to use

I found the lysate to be very effective for our protein studies. The lysate showed a high level of expression and allowed us to obtain consistent results in our Western blot assays.

Q&As

What is the recommended dilution for the SLC6A3/DAT1 lysate in WB?

The optimal dilution of the SLC6A3/DAT1 lysate for WB depends on the specific experimental conditions and the antibody used. Generally, a starting dilution of 1:10 to 1:100 is recommended. It is advisable to perform a dilution series to determine the best concentration for your specific assay. Ensure to include appropriate controls to validate the results.

Can the lysate be used for quantitative analyses, or is it more suitable for qualitative assays?

The SLC6A3/DAT1 lysate can be used for both qualitative and quantitative analyses. For quantitative purposes, it is essential to include a standard curve or known quantity of a control protein to ensure accurate measurements. Proper optimization and calibration are necessary to achieve reliable quantitative results. For qualitative analyses, such as detecting the presence or absence of the DAT1 protein, the lysate is highly suitable.

How to ensure the reproducibility of their results using this lysate?

To ensure reproducibility, researchers should follow standardized protocols for lysate preparation, storage, and usage. Consistent experimental conditions, including antibody concentrations, detection methods, and gel electrophoresis parameters, are also crucial. Running multiple replicates and including appropriate controls can help validate results and identify any inconsistencies. Documenting all procedural details meticulously will aid in reproducing experiments accurately.