Fluoro-Jade B (FJB) Staining Kit, Powder

Name: Fluoro-Jade B (FJB) Staining Kit, Powder
SKU: NRP-0322-P2163
Rating: 5 (1 reviews)

[CAT#: NRP-0322-P2163]

Tracer Type:: Fluorescent Dye

Applications:: Neural Tracing

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Product Overview

Tracer Type

Fluorescent Dye

Applications

Neural Tracing

Application Notes

Following our detailed protocol, Fluoro-Jade C labeled degenerating neurons that were visualized under blue light excitation, while DAPI (not included) counterstained nuclei for visualization under UV light illumination. Fluoro-Jade C dye can be used on a variety of preserved tissues, including fresh-frozen, paraformaldehyde- or formalin-fixed tissue, and formalin-fixed, paraffin-embedded tissue.

Properites

Detection Method

Fluorescence

Excitation/Emission (nm)

FJB visualization is accomplished using blue light or a 488 nm Laser.
Excitation Peak: 495 nm
Emission Peak: 521 nm
Filter system for visualizing: Fluorescein/FITC

Soluble In

Spin vial briefly before opening. See product protocol for detailed use instructions.

Appearance

Dry, Coffee brown to brick red powder; hygroscopic powder keep dessicated.

Shipping

25°C (ambient)

Storage

Powdered dyes can be stored dry at room temperature away from light. Storage in a desiccator is recommended as FJB is hygroscopic. The 0.01% stock solution is stored in the refrigerator for 3 months, protected from light. A 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJB dye solutions are unstable and should not be stored. Other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.